中国冬小麦品质时空变异特征及关键气象因子分析

为精准解析中国冬小麦品质的变异特征及其影响因素，利用地理信息系统(GIS)研究中国冬小麦籽粒品质在2006-2019年的时空分布特征，探究影响冬小麦籽粒品质的关键气象因子，分析冬小麦品质与关键气象因子的空间异质性。结果表明，中国冬小麦籽粒蛋白含量、湿面筋含量和沉淀值在空间上总体呈东北高西南低的变化趋势，各指标在时间上没有一致的变化特征。籽粒蛋白含量与开花至成熟期间最高气温大于30℃的天数呈极显著正相关，空间上具有一定正相关特征；湿面筋含量与开花至成熟期的平均气温呈极显著正相关，空间上具有一定正相关特征；沉淀值与开花至成熟期的日照时数呈显著负相关，空间上呈现出明显的负相关。因此，根据影响冬小麦品质形成的关键气象因子，合理布局冬小麦种植区划，制定冬小麦优质发展的气候适宜区，有利于冬小麦品质提升的气候资源高效利用。     

From regionally predictable to locally complex population structure in a freshwater top predator: river systems are not always the unit of connectivity in Northern Pike Esox lucius

Contemporary genetic diversity is the product of both historical and contemporary forces, such as climatic and geological processes affecting range distribution and continuously moulded by evolutionary forces selection, gene flow and genetic drift. Predatory freshwater fishes, such as Northern Pike Esox lucius, commonly exhibit small population sizes, and several local populations are considered endangered. Pike inhabit diverse habitat types, including lakes, rivers and brackish marine waters, thus spanning from small isolated patches to large open marine systems. However, pike population structure from local to regional scales is relatively poorly described, in spite of its significance to developing conservation measures. We analysed microsatellite variation in a total of 1185 North European pike from 46 samples collected across both local and regional scales, as well as over time, to address two overarching questions: Is pike population structure associated with local and/or regional connectivity patterns, and which factors likely have the main influence on the contemporary distribution of genetic diversity? To answer this, we combined estimators of population diversity and structure to assess evidence of whether populations within (i) habitats, (ii) drainage systems and (iii) geographical regions are closer related than among these ranges, and whether patterns are temporally stable. Contrasting previous predictions that genetic drift obscures signals of postglacial colonisation history, we identified clear regional differences in population genetic signatures, suggesting a major effect of drainage divides on colonisation history and connectivity. However, several populations deviated from the general pattern, showing that local processes may be complex and need to be examined case‐by‐case.     

不同沙丘下梭梭(Haloxylon ammodendron)主根周围土壤特征的差异性研究

为探明沙漠建群种植物个体周围土壤养分和盐分空间分布及成因,对艾比湖湖区边缘三种类型沙丘下梭梭主根周围土壤含水量,电导率,全量养分和速效养分的变化进行了研究。结果表明:1固定性沙丘在表层(0~10 cm)处土壤含水量最小,流动性沙丘和半固定性沙丘在浅层(10~20 cm)处最小,以距离主根面3m处表现最为显著(P0.05)。2流动性沙丘和半固定性沙丘土壤有机质和全氮的结构性和空间自相关性均较弱,固定性沙丘养分(全量养分、速效养分)的结构性则较好。3三种沙丘土壤养分的空间分布不均,主要以条状和斑块状分布,整体上呈现出一定的集聚趋势,其中流动性沙丘和固定性沙丘集聚范围在0~2 m圈层,但纵向集聚范围中,流动性沙丘以浅层(10~20 cm),固定性沙丘以较深层(20~40cm)出现集聚,形成"肥岛"。4流动性沙丘土壤电导率对沙丘的影响较其他各特征因子占主导地位,半固定性沙丘和固定性沙丘养分因子占主导地位。     

生殖细胞早期发生与减数分裂启动调节机制的研究进展

卵巢性生殖疾病发生的主要原因之一是卵子发生过程中减数分裂发生或染色体分离异常。为了更好地进行这些生殖疾病的诊断和治疗,需要充分地了解个体发育的生物学过程中及关键时间点。鉴于此,本文主要阐述了哺乳动物生殖细胞早期发生和减数分裂启动的生理过程,介绍生殖细胞的出现、迁移、性别分化和减数分裂的整个过程。深入了解卵母细胞发生过程将为利用体外发育来源的卵母细胞治疗卵巢性生殖疾病,克服女性不孕、卵巢早衰等重大疾病提供一定的理论支持。     

枸杞岛近岸3种生境鱼类群落组成及岩礁区底栖海藻对鱼类群落结构的影响

2005年—2006年及2010年5—6月对枸杞岛近岸岩礁生境分布的底栖海藻进行观测和潜水采样,发现岩礁生境潮下带底栖海藻组成模式分以大型底栖海藻铜藻为优势种和以孔石莼等小型藻类为优势种两类。2009年2月—2010年1月利用组合刺网对枸杞岛海域岩礁、沙地和贻贝筏式养殖区3种生境的鱼类进行了逐月采样,通过采用方差分析、聚类和非度量多维标度等统计分析手段发现:(1) 岩礁生境鱼类以底栖趋礁杂食性为主,沙地生境鱼类以中下层洄游肉食性为主,贻贝筏式养殖区生境鱼类大型个体占优;(2) 岩礁生境鱼类多样性较高,大型底栖海藻生长的岩礁生境中鱼类群落组成较小型底栖海藻生长的岩礁生境稳定。前者为枸杞岛近岸优势种群繁衍提供有利条件,对鱼类幼体和小型个体有诱集作用,为鱼类捕食者提供更多样化的食物选择,鱼类群落较稳定;后者易受外界鱼类迁移的影响,个体数量和生物量月间差异大。     

长江中游监利江段鱼卵及仔稚鱼时空分布

2010年5-7月对长江中游监利江段鱼卵和仔稚鱼的时空分布进行调查,共采集到鱼卵样品9494粒,仔稚鱼样品328422尾,分属8目15科43属51种.结果显示:5-7月鱼卵的平均密度为13.3 ind./100m3,最大密度值87.8 ind./100 m3,主要集中在5月;5-7月仔稚鱼平均密度为61.8 ind./...     

漳泽水库水环境要素的时空变化特征研究

为阐明漳泽水库水环境要素的时空变化规律,依据历史资料和现场监测进行研究。结果显示,漳泽水库水环境要素年际分布的差异性较大,外源污染物的排放量是影响漳泽水库水质的首要原因。近10年来,漳泽水库水环境要素年际间均呈不显著的上升趋势,但月度变化差异较大;水质指标(BOD5、CODMn和DO)含量月度最高值出现在枯水期的2、3月份;7月份,DO和NH3-N处于最低值。TP在枯水期含量最低,平水期最高,丰水期则居中。温度、降雨量、蓄水量等因子的季节变化对水环境要素年内变化的影响比较显著。水温分层特性增强了水环境要素在垂向的分异性。     

Temporal changes of soil physical quality under two residue management systems

Although crop residue management is known to affect near‐surface soil physical quality, little is known about the temporal variability of these indicators over short time intervals. This study evaluates the temporal changes of nine indicators of soil physical quality. These are organic carbon content, structural stability index, bulk density, macroporosity, air capacity, relative field capacity, plant available water capacity, Dexter's S‐index and saturated hydraulic conductivity. A second set of soil physical indicators, based on the distribution of soil pore volume, was also evaluated. The indicators were determined in three different times during the growing cycle of winter durum wheat cultivated within a long‐term field research carrying out in Southern Italy and comparing two types of crop residue management, that is, burning (B) and soil incorporation (I). Only the bulk density changed over time for both treatments, although the air capacity also changed for the incorporation of wheat residues. Residual effects of the autumnal soil tillage and soil compaction were a common source of variability, irrespective of which treatment was used. Based on the existing guidelines for evaluating the physical quality of these agricultural soils, optimal or near‐optimal values were detected in about half of the cases under consideration. This suggests that both B and I create sufficiently good conditions for crop growth during the crop cycle. The comparison between observed and optimal soil pore distribution function was always poor. The pore volume distributions showed lower densities of small pores and relatively higher densities of large pores than the proposed optimal distribution. This study also suggests that the considered optimal or references curves probably cannot be applied successfully to a wide range of agricultural soils.     

山羊TNNT3基因可变剪切及其对骨骼肌细胞分化的作用

【目的】快速骨骼肌肌钙蛋白T（fast skeletal troponin T3,TNNT3）作为肌钙蛋白(troponin, Tn)家族成员,调节横纹肌收缩、参与骨骼肌的生长发育并影响家畜肉质性状。通过获得山羊TNNT3基因的可变剪切体,分析山羊TNNT3基因可变剪切的表达模式及其在肌细胞分化中的作用,深入解析TNNT3基因在山羊骨骼肌生长发育过程中的作用机制。【方法】基于NCBI已公布山羊TNNT3基因（NM_001314210.1）和牛TNNT3基因（XM_010821200）mRNA序列,使用软件Primer Premier 6.0设计引物,以简州大耳羊胚胎期和出生后7个阶段骨骼肌为试验材料,克隆测序获得山羊TNNT3基因的CDS区可变剪切体,利用软件ORF Finder、EditSeq、DNAMAN、ClustalW和MEGA_X_10.1.8等对序列进行生物信息学分析;进一步设计实时荧光定量（real-time PCR,RT-qPCR）及半定量引物,研究TNNT3基因剪切体在7个不同组织（背最长肌（longissimus dorsi muscle,LD）、半膜肌（semimembranosus muscle,SM）、心、肝、脾、肺、肾）和7个发育阶段（胚胎期E75、E90、E105和出生后B3、B45、B150、B300）肌肉组织（背最长肌和半膜肌）中表达模式;此外,对转录本TNNT3_3进行体外编码能力检测确定其具有编码蛋白的能力,并在山羊骨骼肌卫星细胞（skeletal muscle satellite cells,MuSCs）中过表达,观察细胞形态变化以及检测标志基因的表达变化,研究其对山羊MuSCs分化的作用。【结果】①TNNT3（NM_001314210.1）CDS区全序列主要含有18个外显子,其中外显子16/17相互排斥,转录后单一表达。克隆发现山羊TNNT3基因 5个新转录本（TNNT3_1—5）,其外显子数分别是15、15、20、16、14。②生物信息学分析结果显示山羊TNNT3基因核苷酸序列和氨基酸序列与绵羊、牛、猪等哺乳动物具有很高的一致性,而与鱼类和爬行类动物的一致性较低,说明TNNT3基因序列在哺乳动物高度保守。③TNNT3 mRNA在背最长肌、半膜肌、心、肝、脾、肺、肾7个组织中都有表达,其中在骨骼肌中高度富集(P < 0.01),心脏及肺次之,其余组织中较低;TNNT3 mRNA在背最长肌和半膜肌中的表达始终处于一个动态变化中,胚胎期TNNT3在半膜肌的表达量高于背最长肌（P<0.05）;出生后则背最长肌中高于半膜肌（P<0.05）。④山羊TNNT3基因转录本TNNT3_3重复出现保守的外显子9—11（138bp）,体外翻译实验显示其可编码蛋白且蛋白大小与预期基本相符（37 kD）;相较于对照组,在山羊MuSCs中过表达该转录本使肌分化标志基因Myomaker、MyoG和MyH4 mRNA极显著升高(P < 0.01)。【结论】获得了山羊TNNT3基因具有完整CDS区5个新可变剪切体,TNNT3主要在肌肉组织（背最长肌和半膜肌）中高表达,在哺乳动物中高度保守且促进成肌分化。初步表明TNNT3基因在动物肌肉生长发育中具有重要的生物学功能。